Ten easy steps to conducting transparent, reproducible meta‐analyses for infant researchers

Meta-analyses provide researchers with an overview of the body of evidence in a topic, with quantified estimates of effect sizes and the role of moderators, and weighting studies according to their precision. We provide a guide for conducting a transparent and reproducible meta-analysis in the field of developmental psychology within the framework of the MetaLab platform, in 10 steps: (1) Choose a topic for your meta-analysis, (2) Formulate your research question and specify inclusion criteria, (3) Preregister and document all stages of your meta-analysis, (4) Conduct the literature search, (5) Collect and screen records, (6) Extract data from eligible studies, (7) Read the data into analysis software and compute effect sizes, (8) Visualize your data, (9) Create meta-analytic models to assess the strength of the effect and investigate possible moderators, (10) Write up and promote your meta-analysis. Meta-analyses can inform future studies, through power calculations, by identifying robust methods and exposing research gaps. By adding a new meta-analysis to MetaLab, datasets across multiple topics of developmental psychology can be synthesized, and the dataset can be maintained as a living, community-augmented meta-analysis to which researchers add new data, allowing for a cumulative approach to evidence synthesis

Supporting social innovation through visualisations of community interactions

Online communities that form through the introduction of sociotechnical platforms require significant effort to cultivate and sustain. Providing open, transparent information on community behaviour can motivate participation from community members themselves, while also providing platform administrators with detailed interaction dynamics. However, challenges arise in both understanding what information is conducive to engagement and sustainability, and then how best to represent this information to platform stakeholders. Towards a better understanding of these challenges, we present the design, implementation, and evaluation of a set of simple visualisations integrated into a Collective Awareness Platform for Social Innovation platform titled commonfare.net. We discuss the promise and challenge of bringing social innovation into the digital age, in terms of supporting sustained platform use and collective action, and how the introduction of community visualisations has been directed towards achieving this goal

Thermally-induced lactosylation of whey proteins: Identification and synthesis of lactosylated β-lactoglobulin epitope

The high temperatures used in the production of milk may induce modifications in proteins structure. Due to occurrence of the Maillard reaction, lactose binds lysine residues in proteins, affecting the nutritional value. Milk is also an important source of allergenic proteins (i.e., caseins, β-lactoglobulin and α-lactalbumin). Thus, this modification may also affect the allergenicity of these proteins. Focusing on milk whey proteins, a screening on different Ultra High Temperatures (UHT) and pasteurized milk samples was performed to identify lactosylation sites, in particular in protein known epitopes, and to verify the correlation between lactosylation and the harshness of the treatment. Whey proteins were extracted from milk samples after caseins precipitations at pH 4.6 and, after chymotryptic and tryptic in solution digestion, peptides were analysed by UPLC-MS and LTQ-Orbitrap. Results show the presence of lactosylated lysine residues in several known epitopes. Then, a β-lactoglobulin epitope was selected and synthesized by solid phase synthesis followed by in solution lactosylation, obtaining high reaction yields and purities. The synthesis of lactosylated allergenic epitopes, described here for the first time, is a useful tool for further studies on the technological impacts on food allergenicity

The Secret Garden of Neuronal circRNAs

High-throughput transcriptomic profiling approaches have revealed that circular RNAs (circRNAs) are important transcriptional gene products, identified across a broad range of organisms throughout the eukaryotic tree of life. In the nervous system, they are particularly abundant, developmentally regulated, region-specific, and enriched in genes for neuronal proteins and synaptic factors. These features suggested that circRNAs are key components of an important layer of neuronal gene expression regulation, with known and anticipated functions. Here, we review major recognized aspects of circRNA biogenesis, metabolism and biological activities, examining potential new functions in the context of the nervous system

Growth and lipid class composition of the Arctic pelagic amphipod Themisto libellula

Carnivorous zooplankton is a key element to the energy transfer through the arctic food web, linking lipid rich herbivores to the top predators. We investigated the growth and lipid dynamic of the Arctic pelagic amphipod Themisto libellula in Kongsfjorden (Svalbard, 79°N) from May to October 2007. Additional samplings were performed in spring and summer 2006 and further north in Rijpfjorden (80°N), in September 2006 and 2007. In Kongsfjorden, the first free-swimming stages (3 mm) appeared early May and reached their adult length (25 mm), in October. During their first year, they grew according to a Von Bertalanffy model and most probably constituted a single cohort. Juveniles had the highest growth rate (0.19 mm day−1) and revealed relatively low total lipid (TL) content (about 2.5% wet weight (WW)) with phospholipids as the major lipid class. Sub-adults showed a distinct decrease of growth rates which coincided with the increase of neutral lipid storage, reflecting a switch in energy allocation, from somatic growth to lipid storage. Indeed wax esters (WE) increased up to 48.5% TL on average in adults in 2006 while triacylglycerols (TAG) remained almost constant below 25.2% TL. The absence of lipid accumulation (in disproportion of the weight) in 2007 could be explained by a higher metabolism of T. libellula or preys of lower quality. In Rijpfjorden, adults in their second year continued accumulating lipid (up to 10% WW) with high and similar proportions of both lipid classes, WE and TAG. We highlighted that T. libellula exhibited a variable lipid metabolism along its life cycle depending on its physiological needs and environmental conditions

A novel mutation in SACS gene in a family from southern Italy

A form of autosomal recessive spastic ataxia (ARSACS) has been described in the Charlevoix and Saguenay regions of Quebec. So far a frameshift and a nonsense mutation have been identified in the SACS gene. The authors report a new mutation (1859insC), leading to a frameshift with a premature termination of the gene product sacsin, in two sisters from consanguineous parents. The phenotype is similar to previously described patients with ARSACS